Indolepyruvate pathway for indole-3-acetic acid biosynthesis in Bradyrhizobium elkanii

Kiwamu Minamisawa, Ken Ichiro Ogawa, Hideyuki Fukuhara, Jinichiro Koga

研究成果: Article査読

18 被引用数 (Scopus)

抄録

Indole-3-acetaldehyde (IAAld) was detected in the culture supernatant of Bradyrhizobium elkanii. Deuterium-labelled L-tryptophan (Trp) was incorporated into IAAld and indole-3-acetic acid (IAA), suggesting that B. elkanii produces IAA via IAAld from Trp. In B. elkanii cell suspension, indole-3-pyruvic acid (IPyA) was converted to IAAld, and exogenously added IAAld was rapidly converted to IAA. Furthermore, the activity of indolepyruvate decarboxylase (IPDC), which catalyzes the decarboxylation of IPyA to produce IAAld and is a key enzyme for IPyA pathway, was detected in B. elkanii cell-free extract. The IPDC activity depended on Mg2+ and thiamine pyrophosphate, cofactors of decarboxylation. This mounting evidence strongly suggests that IAA synthesis occurs via IPyA pathway (Trp → IPyA → IAAld → IAA) in B. elkanii.

本文言語English
ページ(範囲)449-453
ページ数5
ジャーナルPlant and Cell Physiology
37
4
DOI
出版ステータスPublished - 1996 6

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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