Independent spatial and temporal functions of human sperm centrosomes after dispermic microinjection into bovine oocytes

Yukihiro Terada, Hisataka Hasegawa, Tomohisa Ugajin, Hiroshi Nabeshima, Kichiya Suzuki, Nobuo Yaegashi, Kunihiro Okamura

研究成果: Article査読

1 被引用数 (Scopus)

抄録

During mammalian fertilization, a centrosome is introduced by the sperm during the first cell cycle to organize a radial array of microtubules known as the sperm aster. In nature, multiple human sperm centrosomes may exist in the same egg cytoplasm during polyspermy. However, critical information concerning individual sperm centrosomal function with regards to the latter case remains unknown. We subsequently examined the sperm aster formation after injection of multiple human sperm into a bovine egg. When 2 fertile human sperm were simultaneously microinjected into different regions of the same bovine egg cytoplasm, no difference in sperm aster formation rate was observed compared to cases in which a single sperm was injected. Two human sperm were also microinjected into bovine eggs 30-, 60- and 120-minute intervals apart from one another, and no difference in sperm aster formation rates were observed. Among eggs in which 1 sperm aster was organized, there was no observable bias towards the first or second injected sperm. These findings indicated that when multiple human sperm are present in a single egg cytoplasm, each centrosome can function independently from the other. This fact suggests the possibility of transplanting a normal sperm centrosome into an egg with a sperm known to have centrosomal dysfunction.

本文言語English
ページ(範囲)559-565
ページ数7
ジャーナルJournal of Andrology
30
5
DOI
出版ステータスPublished - 2009 9

ASJC Scopus subject areas

  • 内分泌学、糖尿病および代謝内科学
  • 生殖医学
  • 内分泌学
  • 泌尿器学

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