Increased hyaluronan production and decreased E-cadherin expression by cytokine-stimulated keratinocytes lead to spongiosis formation

Tomoyuki Ohtani, Ai Memezawa, Ryuhei Okuyama, Tetsuya Sayo, Yoshinori Sugiyama, Shintaro Inoue, Setsuya Aiba

研究成果: Article

37 引用 (Scopus)

抜粋

The pathogenesis of spongiosis, which is a well-known hallmark of acute eczema, is not fully understood. We sought to clarify the mechanism for the influx of tissue fluid into the epidermis and the loss of cohesion between keratinocytes in acute eczema that result in spongiosis. We first demonstrated increased intercellular accumulation of hyaluronan (HA) in the spongiotic epidermis by immunochemical staining using hyaluronic-acid-binding protein (HABP) and augmented hyaluronan synthase 3 (HAS3) mRNA expression by spongiotic keratinocytes using in situ hybridization. We also showed that the epidermis where the intercellular space was strongly stained with HABP showed weaker expression of membrane E-cadherin. Next, we demonstratedby a sandwich assay using HABP, real-time PCR, and flow cytometrythat, among various cytokines, only IL-4, IL-13, and IFN- increased HA production, enhanced HAS3 mRNA expression, and decreased membrane E-cadherin expression by normal human epidermal keratinocytes in both low- and high-Ca media. Finally, we demonstrated IL-4, IL-13, their combination, and IFN- could induce intercellular space widening of the epidermis with increased HA accumulation and decreased E-cadherin expression in the organotypic culture. These results suggest that the augmented production of HA and the decreased E-cadherin expression by keratinocytes stimulated with IL-4IL-13 or IFN- cause spongiosis in acute eczema.

元の言語English
ページ(範囲)1412-1420
ページ数9
ジャーナルJournal of Investigative Dermatology
129
発行部数6
DOI
出版物ステータスPublished - 2009 6

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

フィンガープリント Increased hyaluronan production and decreased E-cadherin expression by cytokine-stimulated keratinocytes lead to spongiosis formation' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用