Increased expression of (pro)renin receptor in the remnant kidneys of 5/6 nephrectomized rats

Takuo Hirose, Nobuyoshi Mori, Kazuhito Totsune, Ryo Morimoto, Takahiro Maejima, Takuya Kawamura, Hirohito Metoki, Kei Asayama, Masahiro Kikuya, Takayoshi Ohkubo, Masahiro Kozuki, Kazuhiro Takahashi, Yutaka Imai

研究成果: Article査読

35 被引用数 (Scopus)

抄録

Recent studies have revealed that (pro)renin receptor ((P)RR), a newly identified member of the renin-angiotensin system, is associated with renal organ damage. However, there is little information regarding the regulation of (P)RR expression in various pathophysiological conditions. We therefore examined the expression of (P)RR in the remnant kidneys of rats with renal mass ablation due to 5/6 nephrectomy by quantitative RT-PCR, Western blot analysis and immunohistochemistry. Expression levels of (P)RR mRNA were significantly increased in the remnant kidneys at day 56 after nephrectomy, when compared with sham operation (about 1.6-fold, P = 0.001). Western blot analysis showed that expression levels of (P)RR protein were greatly increased in the remnant kidneys at day 56, compared with sham operation (about 7.9-fold, P = 0.02). The renal tubular cells were immunostained with anti-(P)RR antibody in both 5/6 nephrectomized rats and sham operated rats. The glomeruli were sporadically immunostained in 5/6 nephrectomized rats, but not in sham operated rats. These findings indicate that the intra-renal (P)RR expression is increased in the remnant kidneys of 5/6 nephrectomized rats, and suggest that (P)RR may contribute to the renal injury.

本文言語English
ページ(範囲)93-99
ページ数7
ジャーナルRegulatory Peptides
159
1-3
DOI
出版ステータスPublished - 2010 1月 8

ASJC Scopus subject areas

  • 生理学
  • 生化学
  • 内分泌学
  • 臨床生化学
  • 細胞および分子神経科学

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