Incorporation of capillary-like structures into dermal cell sheets constructed by magnetic force-based tissue engineering

Kosuke Ino, Akira Ito, Hirohito Kumazawa, Hideaki Kagami, Minoru Ueda, Hiroyuki Honda

研究成果: Article

23 引用 (Scopus)

抜粋

One of the major allenges in tissue engineering remains the construction of vascularized 3D transplants in vitro. We recently proposed novel technologies, termed "magnetic force-based tissue engineering" (Mag-TE), to establish three-dimensional (3D) tissues without using scaffolds. Magnetite cationic liposomes (MCLs), which contain 10-nm magnetite nanoparticles in order to improve accumulation of magnetite nanoparticles in target cells, were used to magnetically label normal human dermal fibroblasts (NHDFs). Magnetically labeled NHDFs were seeded onto ultralow-attachment plates. When a magnet was placed under the plate, cells accumulate on the bottom of the well. After a 24-h-incubation period, the cells form a sheet-like structure, which contains the major dermal extracellular matrix (ECM) components (fibronectin and type I collagen) within the NHDF sheet. Human umbilical vein endothelial cells (HUVECs) were co-cultured with NHDF sheets by two methods: HUVECs and NHDFs were mixed and then allowed to form cell sheets by Mag-TE; or NEW sheets were constructed by Mag-TE and HUVECs were subsequently seeded onto NEW sheets. These methods gave tube-like formation of HAECs, resembling early capillaries, within or on the surface NHDF sheets after short-term 3D co-culture, thus suggesting that Mag-TE may be useful for constructing 3D-tissue involving capillaries.

元の言語English
ページ(範囲)51-58
ページ数8
ジャーナルJOURNAL of CHEMICAL ENGINEERING of JAPAN
40
発行部数1
DOI
出版物ステータスPublished - 2007 1 19

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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