In vivo optical recording of brain interneuron activities from a drosophila male on a treadmill

Soh Kohatsu, Masayuki Koganezawa, Daisuke Yamamoto

    研究成果: Chapter

    抄録

    Recent development of genetically encoded calcium indicators (GECIs) allows us to directly monitor neural activities in in vivo preparations from various organisms, enabling the exploration of neural substrates for complex behaviors. As a showcase for such renovated neuroethology with GECIs, we describe a novel method to record optically interneuron activities from the brain of a tethered male fruit fly Drosophila melanogaster when contact chemosensory stimulation is given to his foreleg. In this protocol, the recording is made on the preparation in which a fly is put on an air-supported styrofoam ball, keeping the moving fly in position to allow monitoring of the activity of central neurons from the fly whose movement is minimally restricted. This protocol is applicable to the analysis of other behaviors induced by stimuli of different modalities with small modifications.

    本文言語English
    ホスト出版物のタイトルGenetically Encoded Functional Indicators
    出版社Humana Press Inc.
    ページ103-112
    ページ数10
    ISBN(印刷版)9781627030137
    DOI
    出版ステータスPublished - 2012

    出版物シリーズ

    名前Neuromethods
    72
    ISSN(印刷版)0893-2336
    ISSN(電子版)1940-6045

    ASJC Scopus subject areas

    • 神経科学(全般)
    • 生化学、遺伝学、分子生物学(全般)
    • 薬理学、毒性学および薬学(全般)
    • 精神医学および精神衛生

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