In vivo induction of prostasin mRNA in colonic epithelial cells by dietary sodium depletion and aldosterone infusion in rats

Kouhei Fukushima, Hiroo Naito, Yuji Funayama, Hitoshi Yonezawa, Sho Haneda, Chikashi Shibata, Iwao Sasaki

研究成果: Article査読

10 被引用数 (Scopus)

抄録

Background: Parallel induction of prostasin, a novel serine protease, together with epithelial sodium channel (ENaC) in the colon, may be essential for physiological response to increased circulating aldosterone. The aim of the present study was to investigate whether aldosterone induces prostasin mRNA in parallel with enhanced expression of ENaC in colonic epithelial cells. Methods: Sprague-Dawley rats were maintained on a sodium-depleted diet or subjected to continuous aldosterone infusion up to 4 weeks. Rats were necropsied at 1, 2, or 4 weeks after the beginning of each treatment. Blood was immediately collected and the large intestine was removed. Plasma aldosterone and arginine-vasopressin (AVP) levels were measured by radio-immunoassay. Epithelial cells were isolated from the right and left colon and RNA was extracted. Expression of prostasin and the α-, β-, and γ-subunits of ENaC was evaluated by quantitative RT-PCR or Northern blot analysis. In another series of experiments, T84 cells were stimulated with aldosterone, dexamethasone, and AVP alone or in combination, and prostasin mRNA was measured by quantitative RT-CPR. Results: Treatment with sodium-depleted diet and a ldosterone infusion resulted in an increase of plasma aldosterone and induction of prostasin mRNA in the left colon. Expression of three subunits of ENaC also increased in the left colon. Induction of prostasin mRNA was observed when T84 cells were stimulated with corticosteroids plus AVP in vitro. Conclusions: Aldosterone has a pivotal role for increasing expression of prostasin in epithelial cells of the left colon. AVP may have a synergistic effect on aldosterone-mediated prostasin induction.

本文言語English
ページ(範囲)940-947
ページ数8
ジャーナルJournal of gastroenterology
39
10
DOI
出版ステータスPublished - 2004 10

ASJC Scopus subject areas

  • 消化器病学

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