In vivo growth suppression of human glioma cells by a 16-mer oligopeptide: A potential new treatment modality for malignant glioma

Katsuhiko Kono, Tetsuya Ueba, Jun A. Takahashi, Nozomu Murai, Nobuo Hashimoto, Akira Myoumoto, Nobuo Itoh, Manabu Fukumoto

研究成果: Article査読

8 被引用数 (Scopus)

抄録

Fibroblast growth factor-2 (FGF-2) is involved as an autocrine growth factor in the autonomous proliferation of glioma cells. To develop a new strategy for treating patients with glioma, we studied the effect on human glioma cells of a 16-mer oligopeptide with conformational similarity to the putative receptor-binding domain of FGF-2. A synthesized oligonucleotide was assessed its receptor-binding activity by BIAcore instrument. Its biological effect on glioma cell lines was examined in vitro by MTT assay. The peptide suppressed the in vitro growth of human glioma cells U87MG, T98G and U251MG cells, but not of A431 cells whose growth is not dependent on FGF-2. Apoptotic bodies were noted after 24-h incubation in the presence of the peptide; Ac-YVAD-CHO, a caspase-3 inhibitor, suppressed apoptosis. Furthermore, we examined the modulation of the cytotoxic effect of anticancer drugs by the oligopeptide. The addition of this oligopeptide to the chemotherapeutic agents CDDP, ACNU and VP16 had additive effects in vitro. These results suggest that the pathway of the FGF-2 autocrine loop through the FGF receptor plays an important role in the proliferation of glioma cells. New drugs targeting this loop may be highly effective in treating FGF-2-dependent tumors. Our results suggest that its addition to the therapeutic arsenal may lead to improved treatment regimens for patients with FGF-2-dependent tumors.

本文言語English
ページ(範囲)163-171
ページ数9
ジャーナルJournal of Neuro-Oncology
63
2
DOI
出版ステータスPublished - 2003 6

ASJC Scopus subject areas

  • 腫瘍学
  • 神経学
  • 臨床神経学
  • 癌研究

フィンガープリント

「In vivo growth suppression of human glioma cells by a 16-mer oligopeptide: A potential new treatment modality for malignant glioma」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル