In vitro plant regeneration in Macroptilium atropurpureum, a legume with a broad symbiont range for nodulation

H. Ezura, N. Nukui, K. I. Yuhashi, K. Minamisawa

研究成果: Article

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Macroptilium atropurpureum is a model legume with a broad symbiont range for nodulation. We have achieved the first in vitro plant regeneration of this species using cv. Siratro. Hypocotyl explants excised from dark-grown seedlings generated slimy, friable calli after three weeks' culture on B5 medium containing 1-2 mg/l kinetin and 0.05 mg/l α-naphthaleneacetic acid. This was followed by the generation of green organogenic callus with shoot buds by subculturing the explants to hormone-free B5 medium 20 days after the start of culture. The green organogenic calli with shoot buds were maintained as organogenic callus by subculturing on the same medium, and shoots were elongated on hormone-free B5 medium. Elongated shoots were rooted on half-strength B5 medium. Most regenerated plants were morphologically normal, diploid and fertile, although tetraploid plants appeared at a low frequency (8%). (C) 2000 Elsevier Science Ireland Ltd.

元の言語English
ページ(範囲)21-27
ページ数7
ジャーナルPlant Science
159
発行部数1
DOI
出版物ステータスPublished - 2000 10 8

ASJC Scopus subject areas

  • Genetics
  • Agronomy and Crop Science
  • Plant Science

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