In vitro induction of human immunodeficiency virus type 1 variants resistant to phosphoralaninate prodrugs of Z-methylenecyclopropane nucleoside analogues

Kazuhisa Yoshimura, Ron Feldman, Eiichi Kodama, Mark F. Kavlick, Yao Ling Qiu, Jiri Zemlicka, Hiroaki Mitsuya

研究成果: Article査読

37 被引用数 (Scopus)

抄録

Two methylenecyclopropane nucleoside analogues with a phenylphosphoralaninate moiety, QYL-685 and QYL-609, exert potent and specific activities against human immunodeficiency virus type 1 strain LAI (HIV-1(LAI)) and HIV-2 in vitro. In this study, we induced HIV-1 variants resistant to QYL-685 by exposing HIV-1(LAI) to increasing concentrations of QYL-685. After 16 passages, the virus (HIV-1(P16)) was less sensitive to QYL- 685 (104-fold), QYL-609 (>41-fold), and (-)-β-2',3'-dideoxy-3'-thiacytidine (3TC) (>1,100-fold) than was HIV-1(LAI) and contained an M184I mutation. Two infectious clones, HIV-1(M184I) and HIV-1(M184V), were resistant to QYL-685, QYL-609, and 3TC, confirming that the M184I mutation was responsible for the observed resistance. Viral-fitness analyses (competitive HIV-1 replication assays) revealed that in the absence of drugs, M184I and M184V conferred a replication disadvantage on the virus compared to the replication efficiency of the wild-type infectious clone (HIV-1(wt)). However, in the presence of QYL-685 (4 μM), HIV-1(M184I) and HIV-1(M184V) showed greater fitness than HIV-l(wt). These data may provide structural and virological relevance with regard to the emergence of M184I and M184V substitutions in HIV-1.

本文言語English
ページ(範囲)2479-2483
ページ数5
ジャーナルAntimicrobial agents and chemotherapy
43
10
DOI
出版ステータスPublished - 1999
外部発表はい

ASJC Scopus subject areas

  • 薬理学
  • 薬理学(医学)
  • 感染症

フィンガープリント

「In vitro induction of human immunodeficiency virus type 1 variants resistant to phosphoralaninate prodrugs of Z-methylenecyclopropane nucleoside analogues」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル