In situ real-time monitoring of biomolecular interactions by using surface infrared spectroscopy

Ayumi Hirano-Iwata, Ryo Taro Yamaguchi, Ko Ichiro Miyamoto, Yasuo Kimura, Michio Niwano

研究成果: Article査読

17 被引用数 (Scopus)

抄録

This paper reviews our recent approaches for in situ label-free detection of biomolecules and their interactions by using infrared absorption spectroscopy (IRAS) in the multiple internal reflection (MIR) geometry. Biomolecular interactions, such as DNA hybridization, DNA hydration, protein-protein interaction, cell growth and cell death, were characterized and monitored in situ both in D2O and H2O media. Combination of MIR-IRAS and various sensing platforms, such as Si, GaAs, porous Si, and porous amunina, was also discussed in terms of sensitivity and applicability to chip analysis. It is demonstrated that MIR-IRAS is a promising tool not only for the label-free detection of biomolecules but also for the accurate discrimination between specific and nonspecific interactions, which is critically important when we are monitoring complex and dynamic biological samples.

本文言語English
論文番号102039
ジャーナルJournal of Applied Physics
105
10
DOI
出版ステータスPublished - 2009

ASJC Scopus subject areas

  • 物理学および天文学(全般)

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