In-situ measurement of photosynthesis using single synecocystis sp. PCC 6803 in a microchamber with gas barrier wall

Hisataka Maruyama, Yu Matsuda, Tomohide Niimi, Nobuyuki Unozumi, Kei Nanatani, F. Arai

    研究成果: Conference contribution

    1 被引用数 (Scopus)

    抄録

    We developed a novel single cell measurement of photosynthesis activity using fluorescent oxygen sensor in a microchamber with gas barrier layer. We used Synechocystis sp. PCC 6803, which is a series of cyanobacteria. The chamber wall contains oxygen sensor and gas barrier layer to measure the dissolved oxygen concentration in the chamber. The oxygen concentration is changed by aerobic respiration and photosynthesis of Synechocystis. The variation of the oxygen concentration was used for evaluation of aerobic respiration and photosynthesis of single Synechocystis. To achieve precise oxygen measurement, we used our proposed compensation of photo-degradation of fluorescence. We demonstrated photosynthesis evaluation of single Synechocystis and calculated the photosynthetic performance of sugar production of single Synechocystis.

    本文言語English
    ホスト出版物のタイトルProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
    出版社Chemical and Biological Microsystems Society
    ページ1570-1572
    ページ数3
    ISBN(印刷版)9780979806452
    出版ステータスPublished - 2012 1月 1
    イベント16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
    継続期間: 2012 10月 282012 11月 1

    出版物シリーズ

    名前Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

    Other

    Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
    国/地域Japan
    CityOkinawa
    Period12/10/2812/11/1

    ASJC Scopus subject areas

    • 化学工学(その他)
    • バイオエンジニアリング

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