Improvement of thermostability of cold-active serine alkaline protease from the psychrotrophic bacterium Shewanella sp. strain Ac10 by rational mutagenesis

Ljudmila Kulakova, Andrey Galkin, Toru Nakayama, Tokuzo Nishino, Nobuyoshi Esaki

研究成果: Article

10 引用 (Scopus)

抜粋

A serine alkaline protease (SapSh) from a psychrophilic bacterium Shewanella sp. strain Ac10 is a cold-active subtilase with low thermostability [Appl. Environ. Microbiol. 65 (1999) 611-617]. By means of homology modeling with other subtilase structures, we have constructed a mutant SapSh containing an extra salt bridge on its surface that exhibits higher thermostability and even higher Vmax/Km,app value than those of the wild-type SapSh.

元の言語English
ページ(範囲)113-117
ページ数5
ジャーナルJournal of Molecular Catalysis B: Enzymatic
22
発行部数1-2
DOI
出版物ステータスPublished - 2003 4 1

ASJC Scopus subject areas

  • Catalysis
  • Bioengineering
  • Biochemistry
  • Process Chemistry and Technology

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