TY - JOUR
T1 - Improved resistance to Vibrio parahaemolyticus in black tiger shrimp Penaeus monodon treated with Streptococcus phocae PI80 and Bacillus subtilis
AU - Pattukumar, V.
AU - Kanmani, P.
AU - Satish Kumar, R.
AU - Yuvaraj, N.
AU - Paari, A.
AU - Arul, V.
N1 - Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2013
Y1 - 2013
N2 - The aim of the study was to establish the immune response induced by Streptococcus phocae PI80, Bacillus subtilis, and commercial probiotics (Uni-Ecosense, EcoForce, Uni-Hatch) in the shrimp, Penaeus monodon. Total hemocyte count, phenoloxidase activity, nitroblue tetrazolium (NBT) reductase assay, phagocytic activity, and disease resistance was evaluated for 30 days. Total hemocyte count, phenoloxidase activity, NBT reductase assay, and phagocytic activity significantly increased in juveniles and adults treated with S. phocae PI80, a mixture of S. phocae PI80 and B. subtilis, or a commercial probiotic, but there were no significant differences in live weight of shrimps treated with probiotics or the control. Treatment with S. phocae PI80 plus B. subtilis enhanced survival of shrimps challenged with Vibrio parahaemolyticus. Our study demonstrates that administration of S. phocae PI80+B. subtilis at 4.6 × 108 CFU/ml water induced immune modulation, enhanced immune ability, and increased resistance to V. parahaemolyticus in juvenile and adult black tiger shrimp.
AB - The aim of the study was to establish the immune response induced by Streptococcus phocae PI80, Bacillus subtilis, and commercial probiotics (Uni-Ecosense, EcoForce, Uni-Hatch) in the shrimp, Penaeus monodon. Total hemocyte count, phenoloxidase activity, nitroblue tetrazolium (NBT) reductase assay, phagocytic activity, and disease resistance was evaluated for 30 days. Total hemocyte count, phenoloxidase activity, NBT reductase assay, and phagocytic activity significantly increased in juveniles and adults treated with S. phocae PI80, a mixture of S. phocae PI80 and B. subtilis, or a commercial probiotic, but there were no significant differences in live weight of shrimps treated with probiotics or the control. Treatment with S. phocae PI80 plus B. subtilis enhanced survival of shrimps challenged with Vibrio parahaemolyticus. Our study demonstrates that administration of S. phocae PI80+B. subtilis at 4.6 × 108 CFU/ml water induced immune modulation, enhanced immune ability, and increased resistance to V. parahaemolyticus in juvenile and adult black tiger shrimp.
KW - Commercial probiotics
KW - Immune response
KW - Penaeus monodon
KW - Probiotics
KW - Streptococcus phocae
KW - Vibrio parahaemolyticus
UR - http://www.scopus.com/inward/record.url?scp=84876192732&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84876192732&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:84876192732
VL - 65
JO - Bamidgeh
JF - Bamidgeh
SN - 0792-156X
IS - 1
ER -