We observed that Lgr4K5 KO mice had sparse head hair and focal alopecia behind their ears, as observed in tabby (Eda) and downless (Edar) mice, which are models of the human genetic disorder hypohidrotic (anhidrotic) ectodermal dysplasia (HED). Lgr4-deficient mice showed partial impairment in hair follicle development with reduced expression of Edar, Lef1, and SAA, which were essential for hair follicle morphogenesis, in the epidermis. Immunohistochemical analysis of Lgr4-/- mice epidermis using shh antibody showed reduced numbers of hair placodes, and we also detected higher phosphorylation of Smadl/5/8, which is required to suppress normal hair follicle induction. We suspected that Lgr4 might be a novel gene class regulating the development of hair follicles.
ASJC Scopus subject areas
- Developmental Biology