Impact in stability during sequential CDR grafting to construct camelid VHH antibodies against zinc oxide and gold

Ryota Saito, Yutaro Saito, Hikaru Nakazawa, Takamitsu Hattori, Izumi Kumagai, Mitsuo Umetsu, Koki Makabe

研究成果: Article査読

1 被引用数 (Scopus)

抄録

Biomolecules which recognize inorganic materials and metal surfaces gain much attention for creating new type of nanomaterials and sensors. 4F2, a camelid VHH antibody, recognizes ZnO surface and has been applied for sensor applications. 4F2 was constructed sequential complementarity determining region (CDR) replacement on the parental VHH antibody, termed the Construction of Antibody by Integrating Grafting and Evolution Technology; CAnIGET procedure. Here, we evaluate the influence of CDR replacements during 4F2 generation using calorimetric technique. We found that the initial peptide grafting at CDR1 results in the stability reduction and subsequent CDR3 randomize and selection restore the stability during the construction of 4F2. Further examination using anti-gold VHH, AuE32, revealed that the final CDR3 randomize and selection step has little effect in stability while the initial CDR1 grafting reduces the stability as same as the case for 4F2. Our results showing here provide the detailed view of the stability alteration during the CAnIGET procedure.

本文言語English
ページ(範囲)21-25
ページ数5
ジャーナルJournal of biochemistry
164
1
DOI
出版ステータスPublished - 2018 7 1

ASJC Scopus subject areas

  • 生化学
  • 分子生物学

フィンガープリント

「Impact in stability during sequential CDR grafting to construct camelid VHH antibodies against zinc oxide and gold」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル