Immunolocalization of aromatase, 17α-hydroxylase and side-chain-cleavage cytochromes P-450 in the human ovary

H. Sasano, M. Okamoto, J. I. Mason, E. R. Simpson, C. R. Mendelson, N. Sasano, S. G. Silverberg

研究成果: Article査読

173 被引用数 (Scopus)

抄録

Immunohistochemical localization of cholesterol side-chain-cleavage, 17α-hydroxylase and aromatase cytochromes P-450 was performed in 35 morphologically normal human premenopausal ovaries by using specific antibodies against the enzymes. In well-developed ovarian follicles in the late stages of follicular growth, immunoreactivity of P-450(AROM) was only seen in granulosa cells while P-450(17α) and P-450(SCC) activity was confined to theca interna cells, confirming that follicular oestrogen is produced in granulosa cells by the aromatization of androgens derived from the theca interna cells. In the corpus luteum, this functional differentiation is maintained, since immunoreactivity of P-450(AROM) was exclusively present in luteinized granulosa cells while that of P-450(17α) was present in luteinized theca cells. Immunoreactivity of P-450(SCC) was present in both types of cells in the corpus luteum.

本文言語English
ページ(範囲)163-169
ページ数7
ジャーナルJournal of Reproduction and Fertility
85
1
出版ステータスPublished - 1989

ASJC Scopus subject areas

  • 生理学
  • 胎生学
  • 分子生物学
  • 産婦人科学
  • 発生生物学

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