Immunohistochemical analysis of cytochrome P-450 17α-hydroxylase in pig adrenal cortex, testis and ovary

Sasano Hironobu, J. Ian Mason, Sasano Nobuaki

研究成果: Article査読

32 被引用数 (Scopus)

抄録

Cytochrome P-450 specific for steroid 17α-hydroxylation (P-45017α) was immunohistochemically observed in pig adrenal cortex, testis and ovary by the biotin-streptavidin method using a specific antibody against P-45017α purified from neonatal pig testis. In the adrenal cortex, P-45017α was present in the zona fasciculata and reticularis while no immunoreactivity was observed in the zona glomerulosa, confirming the absence of 17-hydroxylated steroid synthesis in the zona glomerulosa. In the testis, P-45017α was present exclusively in Leydig cells and immunoreactivity was absent in seminiferous tubules. In the ovary, immunoreactivity was observed only in the theca interna but not in the membrana granulosa of follicles. Among the tissues examined, the relative intensity of immunoreactivity was greatest in the Leydig cells, and progressively less in theca interna cells, outer fasciculata cells and inner fasciculata and reticularis cells.

本文言語English
ページ(範囲)197-202
ページ数6
ジャーナルMolecular and Cellular Endocrinology
62
2
DOI
出版ステータスPublished - 1989 4

ASJC Scopus subject areas

  • 生化学
  • 分子生物学
  • 内分泌学

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