Immunohistochemical analysis-based proteomic subclassification of newly diagnosed glioblastomas

Kazuya Motomura, Atsushi Natsume, Reiko Watanabe, Ichiro Ito, Yukinari Kato, Hiroyuki Momota, Ryo Nishikawa, Kazuhiko Mishima, Yoko Nakasu, Tatsuya Abe, Hiroki Namba, Yoichi Nakazato, Hiroshi Tashiro, Ichiro Takeuchi, Tsutomu Mori, Toshihiko Wakabayashi

研究成果: Article査読

39 被引用数 (Scopus)

抄録

Recent gene expression and copy number profilings of glioblastoma multiforme (GBM) by The Cancer Genome Atlas (TCGA) Research Network suggest the existence of distinct subtypes of this tumor. However, these approaches might not be easily applicable in routine clinical practice. In the current study, we aimed to establish a proteomics-based subclassification of GBM by integrating their genomic and epigenomic profiles. We subclassified 79 newly diagnosed GBM based on expression patterns determined by comprehensive immunohistochemical observation in combination with their DNA copy number and DNA methylation patterns. The clinical relevance of our classification was independently validated in TCGA datasets. Consensus clustering identified the four distinct GBM subtypes: Oligodendrocyte Precursor (OPC) type, Differentiated Oligodendrocyte (DOC) type, Astrocytic Mesenchymal (AsMes) type and Mixed type. The OPC type was characterized by highly positive scores of Olig2, PDGFRA, p16, p53 and synaptophysin. In contrast, the AsMes type was strongly associated with strong expressions of nestin, CD44 and podoplanin, with a high glial fibrillary acidic protein score. The median overall survival of OPC-type patients was significantly longer than that of the AsMes-type patients (19.9 vs 12.8 months). This finding was in agreement with the Oncomine analysis of TCGA datasets, which revealed that PDGFRA and Olig2 were favorable prognostic factors and podoplanin and CD44 were associated with a poor clinical outcome. This is the first study to establish a subclassification of GBM on the basis of immunohistochemical analysis. Our study will shed light on personalized therapies that might be feasible in daily neuropathological practice.

本文言語English
ページ(範囲)1871-1879
ページ数9
ジャーナルCancer science
103
10
DOI
出版ステータスPublished - 2012 10月
外部発表はい

ASJC Scopus subject areas

  • 腫瘍学
  • 癌研究

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