Immuno-northern blotting: Detection of modified RNA using gel separation and antibodies to modified nucleosides

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Immuno-northern blotting is a method for detecting modified RNAs using gel separation and specific antibodies to modified nucleosides. This method was developed by combining two commonly used molecular biology techniques: western blotting and northern blotting. In this method, urea-polyacrylamide (or agarose) gel-separated RNAs are transferred to positively charged nylon membrane and then immune detection is performed with specific antibodies to modified nucleosides: such as 1-methyladenosine, N6-methyladenosine, and pseudouridine. This highly sensitive and relatively simple method, which uses widely available laboratory equipment, enables small laboratories to compare the abundance of modified nucleic acids across samples.

元の言語English
ホスト出版物のタイトルMethods in Molecular Biology
出版者Humana Press Inc.
ページ179-187
ページ数9
DOI
出版物ステータスPublished - 2019

出版物シリーズ

名前Methods in Molecular Biology
1870
ISSN(印刷物)1064-3745

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • これを引用

    Mishima, E., & Abe, T. (2019). Immuno-northern blotting: Detection of modified RNA using gel separation and antibodies to modified nucleosides. : Methods in Molecular Biology (pp. 179-187). (Methods in Molecular Biology; 巻数 1870). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8808-2_13