Imaging of enzyme activity by scanning electrochemical microscope equipped with a feedback control for substrate-probe distance

Daisuke Oyamatsu, Yu Hirano, Norihiro Kanaya, Yoshiaki Mase, Matsuhiko Nishizawa, Tomokazu Matsue

研究成果: Article査読

41 被引用数 (Scopus)

抄録

The enzymatic activity of diaphorase (Dp) immobilized on a solid substrate was characterized using a scanning electrochemical microscope (SECM) with shear force feedback to control the substrate-probe distance. The shear force between the substrate and the probe was monitored with a tuning fork-type quartz crystal and used as the feedback control to set the microelectrode probe close to the substrate surface. The sensitivity and the contrast of the SECM image were improved in the constant distance mode (distance, 50 nm) with the shear force feedback compared to the image in the constant height mode without the feedback. By using this system, the SECM and topographic images of the immobilized diaphorase were simultaneously measured. The microelectrode tip used in this study was ground aslant like a syringe needle in order to obtain the shaper topographic images. This shape was also effective for avoiding the interference during the diffusion of the enzyme substrates.

本文言語English
ページ(範囲)115-121
ページ数7
ジャーナルBioelectrochemistry
60
1-2
DOI
出版ステータスPublished - 2003 8

ASJC Scopus subject areas

  • 生物理学
  • 物理化学および理論化学
  • 電気化学

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