Imaging of caspase-3 activation in HeLa cells stimulated with etoposide using a novel fluorescent probe

Shin Mizukami, Kazuya Kikuchi, Tsunehiko Higuchi, Yasuteru Urano, Tetsuo Mashima, Takashi Tsuruo, Tetsuo Nagano

研究成果: Article査読

102 被引用数 (Scopus)

抄録

Microscopic visualization of intracellular enzyme activity can provide information about the physiological role of the enzyme. Caspases are cysteine proteases that have critical roles in the execution of apoptosis. General fluorometric substrates of caspase-3, such as DEVD-MCA, are unsuitable for imaging because they are excited at short wavelength, so we designed and synthesized novel fluorescent probes that are excited at suitable wavelengths for detecting caspase-3 activity in living cells. Using one of these probes, we succeeded in microscopic visualization of caspase-3-like activity within HeLa cells treated with etoposide. The caspase-3-like activity was increased in the cytosol at first, then expanded to the whole cell.

本文言語English
ページ(範囲)356-360
ページ数5
ジャーナルFEBS Letters
453
3
DOI
出版ステータスPublished - 1999 6 25
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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