Identification of trf2 mutants of Drosophila with defects in anterior spiracle eversion

Seigo Shima, Toshiro Aigaki, Tetsuya Nojima, Daisuke Yamamoto

    研究成果: Article査読

    5 被引用数 (Scopus)

    抄録

    TATA-box-binding protein (TBP)-related factor (Trf)2 is a member of the family of TBP-related factors present in metazoan organisms. In Drosophila, Trf2 immunoprecipitates with the nucleosome remodeling factor (NURF) chromatin remodeling complex and the DNA replication element (DRE)-binding factor DREF. When it forms a complex with DREF, Trf2 activates transcription from the DRE-binding sites of the proliferating cell nuclear antigen (PCNA) gene. Despite these observations at the molecular level, no mutations in the trf2 locus have been found in Drosophila. Here, we identify two P-element insertion alleles, PL28 and GS7403, as hypomorphic mutants with a decreased expression level of trf2. Pupae of these mutant alleles show failure in anterior spiracle eversion, a hallmark of mutations in the loci associated with ecdysteroid signaling.

    本文言語English
    ページ(範囲)157-163
    ページ数7
    ジャーナルArchives of Insect Biochemistry and Physiology
    64
    4
    DOI
    出版ステータスPublished - 2007 4月 1

    ASJC Scopus subject areas

    • 生理学
    • 生化学
    • 昆虫科学

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