Identification of transcripts expressed preferentially in hemocytes of Ciona intestinalis that can be used as molecular markers

Takeshi Wakoh, Makoto Ikeda, Ryuji Uchino, Kaoru Azumi, Masaru Nonaka, Yuji Kohara, Hitoe Metoki, Yutaka Satou, Nori Satoh, Masanobu Satake

研究成果: Article査読

14 被引用数 (Scopus)

抄録

The immunity provide by ascidian hemocytes represents one prototype of innate immune function in vertebrates. However, there are currently no molecular markers of ascidian hemocytes. We accumulated a large number of ESTs of cDNAs derived from hemocytes of Ciona intestinalis, a cosmopolitan species of ascidian. By comparing these ESTs with those derived from other tissues and developmental stages of Ciona, we were able to extract 81 transcripts expressed abundantly and preferentially in hemocytes. Among them, the von Willebrand factor type A (vWA)-like and complement 6 (C6)-like transcripts were found to be expressed almost exclusively in hemocytes, based on RT-PCR analysis and whole mount in situ hybridization. We propose that vWA-like and C6-like can be used as molecular markers for Ciona hemocytes.

本文言語English
ページ(範囲)345-352
ページ数8
ジャーナルDNA Research
11
5
DOI
出版ステータスPublished - 2004

ASJC Scopus subject areas

  • 分子生物学
  • 遺伝学

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