Identification of three commonly deleted regions on chromosome arm 6q in human pancreatic cancer

Abe Tadayoshi, Naohiko Makino, Toru Furukawa, Hong Ouyang, Mitsuhiro Kimura, Toshimasa Yatsuoka, Tadaaki Yokoyama, Hiroko Inoue, Shinichi Fukushige, Masato Hoshi, Yutaka Hayashi, Makoto Sunamura, Masao Kobari, Seiki Matsuno, Akira Horii

研究成果: Article査読

30 被引用数 (Scopus)

抄録

Pancreatic cancer has one of the poorest prognoses among malignant diseases. To understand its molecular mechanisms, we studied allelic losses on the long arm of chromosome 6. Using 55 paired DNAs of tumors and their corresponding normal tissues and 30 microsatellite markers that spanned the entire 6q chromosome arm, we found three distinct regions of common allelic loss: region A, a less than 500-kb region bordered by D65449 and D65283 on 6q21 with a loss of heterozygosity (LOH) frequency of 69% (38/55); region B, a 7-cM region bordered by D65292 and D65308 on 6q23-q24 with a LOH frequency of 60% (33/55); and region C, a 13-cM region bordered by D65305 and D65264 with a LOH frequency of 51% (28/55). We further focused on region A and constructed a physical map using yeast artificial chromosome (YAC) clones, their derived cosmid clones, and bacterial artificial chromosome (BAC) clones. Region A was completely covered by three overlapping BAC clones. Our results in the present study should shed light on the cloning and characterization of tumor suppressor genes in pancreatic carcinogenesis.

本文言語English
ページ(範囲)60-64
ページ数5
ジャーナルGenes Chromosomes and Cancer
25
1
DOI
出版ステータスPublished - 1999 5 1

ASJC Scopus subject areas

  • 遺伝学
  • 癌研究

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