Identification of substrates of F-box protein involved in methylmercury toxicity in yeast cells

Jin Yong Lee, Yosuke Ishida, Shusuke Kuge, Akira Naganuma, Gi Wook Hwang

研究成果: Article査読

8 被引用数 (Scopus)

抄録

We previously reported that some of the substrate proteins recognized by Hrt3 or Ucc1, a component of Skp1/Cdc53/F-box protein ubiquitin ligase, may include proteins that are involved in the methylmercury toxicity and degraded by the proteasome. In this study, we found that Dld3 and Grs1 bound to Hrt3 and that Eno2 bound to Ucc1 using a yeast two-hybrid screening. We demonstrated that Dld3 and Grs1 are substrates that are ubiquitinated by Hrt3, and Eno2 is a substrate that is ubiquitinated by Ucc1. Moreover, any yeast showing overexpression of Dld3, Grs1, and Eno2 demonstrated higher methylmercury sensitivity. This indicates that Hrt3 and Ucc1 are involved in alleviating the methylmercury toxicity by promoting proteasomal degradation through the ubiquitination of Dld3, Grs1, and Eno2.

本文言語English
ページ(範囲)2720-2725
ページ数6
ジャーナルFEBS Letters
589
19
DOI
出版ステータスPublished - 2015 9 14

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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