Identification of SATB1 as a specific biomarker for lymph node metastasis in colorectal cancer

Hironobu Baba, Toshiaki Ishikawa, Kaoru Mogushi, Megumi Ishiguro, Hiroyuki Uetake, Hiroshi Tanaka, Kenichi Sugihara

研究成果: Article

6 引用 (Scopus)

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Background/Aim: The integration of gene expression analysis and genomic profiling represents an efficient approach to the discovery of cancer-related genes. Lymph node metastasis (LNM) is a significant prognostic factor in colorectal cancer (CRC). Detection and analysis of factors related to LNM will help develop new diagnostic methods or therapies. In this study, we aimed to identify genes that are significantly related to LNM in CRC through integrated copy number analysis (CNA) and gene expression analysis. Materials and Methods: Genes showing both upregulated expression and copy number gains in cases involving CRC with LNM were extracted as candidate biomarkers. Expression of the mRNA of the final candidate was validated in 124 patients using quantitative reverse transcription polymerase chain reaction (qRT-PCR) assays. Expression of the protein encoded by this candidate gene was assessed using immunohistochemical (IHC) staining of tissue from 328 patients. The association between protein expression and clinicopathological features was also examined. Results: Special AT-rich sequence-binding protein 1 (SATB1) was extracted from integrated microarray analysis. SATB1 mRNA expression in cancer tissue was significantly higher in patients with LNM than without LNM. SATB1 protein overexpression was significantly associated with LNM. Moreover, overexpression of SATB1 was an independent poor prognostic factor in stage I-III, especially in stage II CRC. Conclusion: SATB1 may play an important role in LNM of CRC. SATB1 may be a biomarker of LNM and of recurrence after surgery for CRC.

元の言語English
ページ(範囲)4069-4072
ページ数4
ジャーナルAnticancer research
36
発行部数8
出版物ステータスPublished - 2016 8

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

フィンガープリント Identification of SATB1 as a specific biomarker for lymph node metastasis in colorectal cancer' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

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    Baba, H., Ishikawa, T., Mogushi, K., Ishiguro, M., Uetake, H., Tanaka, H., & Sugihara, K. (2016). Identification of SATB1 as a specific biomarker for lymph node metastasis in colorectal cancer. Anticancer research, 36(8), 4069-4072.