Identification of regions in which positive selection may operate in S-RNase of Rosaceae: Implication for S-allele-specific recognition sites in S-RNase

Takeshi Ishimizu, Toshinori Endo, Yumi Yamaguchi-Kabata, Kazuo T. Nakamura, Fumio Sakiyama, Shigemi Norioka

研究成果: Article査読

79 被引用数 (Scopus)

抄録

A stylar S-RNase is associated with gametophytic self-incompatibility in the Rosaceae, Solanaceae, and Scrophulariaceae. This S-RNase is responsible for S-allele-specific recognition in the self-incompatible reaction, but how it functions in specific discrimination is not clear. Window analysis of the numbers of synonymous (d(S)) and non-synonymous (d(N)) substitutions in rosaceous S-RNases detected four regions with an excess of d(N) over d(S) in which positive selection may operate (PS regions). The topology of the secondary structure of the S-RNases predicted by the PHD method is very similar to that of fungal RNase Rh whose tertiary structure is known. When the sequences of S-RNases are aligned with the sequence of RNase Rh based on the predicted secondary structures, the four PS regions correspond to two surface sites on the tertiary structure of RNase Rh. These findings suggest that in S-RNases the PS regions also form two sites and are candidates for the recognition sites for S-allele-specific discrimination. Copyright (C) 1998 Federation of European Biochemical Societies.

本文言語English
ページ(範囲)337-342
ページ数6
ジャーナルFEBS Letters
440
3
DOI
出版ステータスPublished - 1998 12 4

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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