Identification of protein tyrosine phosphatases expressed in murine male germ cells

Yoshiyuki Kaneko, Seiichiro Takano, Kazuhiro Okumura, Jun Takenawa, Hiroaki Higashituji, Manabu Fukumoto, Hiroki Nakayama, Jun Fujita

研究成果: Article査読

23 被引用数 (Scopus)

抄録

We cloned five cDNAs encoding putative protein tyrosine phosphatases (PTPs; protein-tyrosine-phosphatase phosphohydrolase, EC 3.1.3.48) from the murine testis by using degenerate primers and the polymerase chain reaction cloning technique. Two of them were identical to the mouse cytoplasmic PTP and PTP-1B. The remaining three were likely to represent the murine counterparts of human FTP δ, human FTP ε(lunate), and rat striatum-enriched PTP. The cells expressing these genes were determined either by in situ hybridization histochemistry or by Northern blot hybridization. Moreover, Northern blot hybridization revealed that the transcripts of FTP-1B, mouse cytoplasmic FTP and the murine homologue of human PTP δ were quantitatively and/or structurally regulated during germ cell development, suggesting their roles in spermatogenesis.

本文言語English
ページ(範囲)625-631
ページ数7
ジャーナルBiochemical and biophysical research communications
197
2
DOI
出版ステータスPublished - 1993 12 15

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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