Identification of pin1-binding phosphorylated proteins in the mouse brain

Yota Tatara, Takahiro Terakawa, Takafumi Uchida

研究成果: Article査読

5 被引用数 (Scopus)

抄録

To determine the role of Pin1 in the neurotransmission pathway, Pin1-binding proteins in mouse brain extract were identified. The Pin1-binding proteins were extracted from mouse brain homogenate, and the trypsin-digested peptides were analyzed by nano-liquid chromatography tandem mass spectrometry (LC-MS/ MS). Proteins that involve the neurotransmission pathway, such as synapsin I, synapsin II, and calcium/ calmodulin-dependent protein kinase type II (CaMKII), were identified in a Mascot search. Pull-down and immunoprecipitation assay indicated that Pin1 binds CaMKII in a phosphorylation-specific manner. It was assumed that Pin1 participates in the neurotransmission pathway involving the phosphorylation signal by CaMKII.

本文言語English
ページ(範囲)2480-2483
ページ数4
ジャーナルBioscience, Biotechnology and Biochemistry
74
12
DOI
出版ステータスPublished - 2010 12 1

ASJC Scopus subject areas

  • バイオテクノロジー
  • 生化学
  • 分子生物学
  • 応用微生物学とバイオテクノロジー
  • 分析化学
  • 有機化学

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