抄録
The gene expression profile of human cord blood-derived mast cells (MCs) was investigated using serial analysis of gene expression (SAGE). A total of 22914 tags, representing 9181 unique transcripts, were sequenced. By selecting tags that were detected more frequently in MCs than in other tissues, genes characteristic of MCs were enriched. Reverse transcription-PCR and the high-density oligonucleotide array hybridization confirmed the validity of our SAGE result. About 70% of the selected genes were previously uncharacterized. Northern blot analysis showed the MC-specific expression of selected genes. This inventory will be useful to identify novel genes with important functions in MCs.
| 本文言語 | English |
|---|---|
| ページ（範囲） | 37-41 |
| ページ数 | 5 |
| ジャーナル | FEBS Letters |
| 巻 | 498 |
| 号 | 1 |
| DOI | |
| 出版ステータス | Published - 2001 6月 1 |
| 外部発表 | はい |
ASJC Scopus subject areas
- 生物理学
- 構造生物学
- 生化学
- 分子生物学
- 遺伝学
- 細胞生物学