Identification of N-arachidonylglycine, U18666A, and 4-androstene-3,17- dione as novel insulin Secretagogues

Yukio Ikeda, Haruhisa Iguchi, Masanori Nakata, Ryoichi X. Ioka, Toshiya Tanaka, Satoshi Iwasaki, Kenta Magoori, Shinobu Takayasu, Tokuo T. Yamamoto, Tatsuhiko Kodama, Toshihiko Yada, Takeshi Sakurai, Masashi Yanagisawa, Juro Sakai

研究成果: Article査読

26 被引用数 (Scopus)

抄録

The glucose-induced insulin secretion is fine-tuned by numerous factors. To systematically identify insulinotropic factors, we optimized a primary β-cell-based functional assay to monitor intracellular Ca2+ flux ([Ca2+]i). By this assay system, we successfully identified several insulinotropic peptides including cholecystokinin, gastrin releasing peptide, vasopressin, and oxytocin from tissue extracts. Screening of an assortment of chemical compounds, we determined three novel insulin secretagogues: N-arachidonylglycine (NAGly), 3β-(2-diethylamino-ethoxy) androstenone hydrochloride (U18666A), and 4-androstene-3,17-dione. The NAGly increased [Ca2+]i through stimulation of the voltage-dependent Ca2+ channels and it was dependent on extracellular glucose level. On the other hand, U18666A and 4-androstene-3,17-dione increased [Ca2+]i in the presence of KATP channel opener diazoxide while it was inhibited by the presence of Ca2+ channel blocker nitrendipine, suggesting that their effects are independent of K ATP channel. These unique features will be useful for further development of insulinotropic factors and drugs for treating type 2 diabetes.

本文言語English
ページ(範囲)778-786
ページ数9
ジャーナルBiochemical and biophysical research communications
333
3
DOI
出版ステータスPublished - 2005 8 5

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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