Identification of androgen-responsive microRNAs and androgen-related genes in breast cancer

Kiichiroh Nakano, Yasuhiro Miki, Shuko Hata, Akiko Ebata, Kiyoshi Takagi, Keely May Mcnamara, Minako Sakurai, Mariko Masuda, Hisashi Hirakawa, Takanori Ishida, Takashi Suzuki, Noriaki Ohuchi, Hironobu Sasano

研究成果: Article査読

13 被引用数 (Scopus)

抄録

Background: Androgen receptors (ARs) are expressed in many breast cancer cells, but the mechanism of action of androgens is not as well-characterized as in other cell types. The study of microRNAs has recently provided with important insights into the biology of hormonedependent cancer. Materials and Methods: We attempted to identify microRNAs induced by dihydrotestosterone in an ARpositive cell line. We examined a possible correlation among microRNAs, target genes, and ARs in breast cancer tissues using immunohistochemistry and laser capture microscopy. Results: Our analysis demonstrated that miR-363 and its possible target IQ motif and WD repeats-1 (IQWD1) are involved in a microRNA-mRNA pathway related to the mechanism of action of androgens. Our analyses showed that a high tumor level of IQWD1 in patients with breast cancer was significantly associated with adverse clinical outcomes. Conclusion: Our findings indicated that the recruitment of IQWD1 to ARs may be a prerequisite for the growth stimulation by androgens through ARs in breast cancer cells.

本文言語English
ページ(範囲)4811-4820
ページ数10
ジャーナルAnticancer research
33
11
出版ステータスPublished - 2013 11

ASJC Scopus subject areas

  • 腫瘍学
  • 癌研究

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