Identification of a novel conjugate in human urine: Bile acid acyl galactosides

Takaaki Goto, Akihiro Shibata, Daisuke Sasaki, Naoto Suzuki, Takanori Hishinuma, Genta Kakiyama, Takashi Iida, Nariyasu Mano, Junichi Goto

研究成果: Article査読

27 被引用数 (Scopus)

抄録

We report a novel conjugate, bile acid acyl galactosides, which exist in the urine of healthy volunteers. To identify the two unknown peaks obtained in urine specimens from healthy subjects, the specimens were subjected to solid phase extraction and then to liquid chromatographic separation. The eluate corresponding to the unknown peaks on the chromatogram was collected. Following alkaline hydrolysis and liquid chromatography (LC)/electrospray ionization (ESI)-mass spectrometric (MS) analysis, cholic acid (CA) and deoxycholic acid (DCA) were identified as liberated bile acids. When a portion of the alkaline hydrolyzate was subjected to a derivatization reaction with 1-phenyl-3-methyl-5- pyrazolone, a derivative of galactose was detected by LC/ESI-MS. Finally, the liquid chromatographic and mass spectrometric properties of these unknown compounds in urine specimens were compared to those of authentic specimens and the structures were confirmed as CA 24-galactoside and DCA 24-galactoside. These results strongly imply that bile acid 24-galactosides, a novel conjugate, were synthesized in the human body.

本文言語English
ページ(範囲)185-192
ページ数8
ジャーナルSteroids
70
3
DOI
出版ステータスPublished - 2005 3

ASJC Scopus subject areas

  • 生化学
  • 分子生物学
  • 内分泌学
  • 薬理学
  • 臨床生化学
  • 有機化学

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