Identification of a Genetic Factor Required for High γ-Isoform Concentration in Rice Vitamin E

Daisuke Sekine, Kazumasa Murata, Toshiyuki Kimura, Kiyotaka Nakagawa, Teruo Miyazawa

研究成果: Article査読

3 被引用数 (Scopus)

抄録

The γ-isoforms of tocopherols (Tc) and tocotrienols (T3) possess high biological activities in comparison to the α-isoforms. The concentrations of Tc and T3 isoforms in rice (Oriza sativa) was cultivar-dependent. Using chromosome segment substitution lines (CSSLs) and near isogenic lines (NILs) of indica cultivar "Kasalath" in a japonica cultivar "Koshihikari" genetic background, the Kasalath genomic segment on chromosome 2 was determined to be responsible for the high γ-isoform concentration: γ-tocopherol methyltransferase (γ-TMT) was identified as a candidate gene. An amino acid substitution in the coding region and several nucleotide polymorphisms, including an insertion of 10 base pairs in the promoter region, were identified. Gene expression analysis revealed that low expression levels of the γ-TMT gene in Kasalath were not associated with the γ-isoform concentration. Genetic variations in the coding region of the γ-TMT gene may play a major role in determining the γ-isoform concentration. This information could be used to breed rice with a high γ-isoform content.

本文言語English
ページ(範囲)9368-9373
ページ数6
ジャーナルJournal of Agricultural and Food Chemistry
64
49
DOI
出版ステータスPublished - 2016 12 14

ASJC Scopus subject areas

  • 化学 (全般)
  • 農業および生物科学(全般)

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