Identification of a cardiac isoform of the murine calcium channel α1C (Cav1.2-a) subunit and its preferential binding with the β2 subunit

Manabu Murakami, Takayoshi Ohba, Yoichiro Takahashi, Hiroyuki Watanabe, Ichiro Miyoshi, Shinsuke Nakayama, Kyoichi Ono, Hiroshi Ito, Toshihiko Iijima

研究成果: Article査読

4 被引用数 (Scopus)

抄録

We describe a cardiac muscle isoform of the voltage-dependent calcium channel α1 subunit, which corresponds to the rabbit ortholog of α1C-a (Cav1.2a). We also cloned smooth muscle isoforms α1C-b (Cav1.2b) and α1C-d (Cav1.2d). Differences among these three isoforms lie within the N-terminal region (exon 1A or 1B), the sixth transmembrane segment of domain I (exon 8A or 8B), and the use of exon 10, which forms the intracellular loop between transmembrane domains I and II. Two-hybrid analysis revealed interactions among the three α1 isoforms and β subunits. In vitro overlay and immunoprecipitation analyses revealed preferential binding between α1C-a and β2, which is also expressed at a high level in the heart.

本文言語English
ページ(範囲)115-125
ページ数11
ジャーナルJournal of Molecular and Cellular Cardiology
41
1
DOI
出版ステータスPublished - 2006 7
外部発表はい

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

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