Identification and evaluation of anti-inflammatory compounds from Kaempferia parviflora

Satoru Horigome, Izumi Yoshida, Aiko Tsuda, Teppei Harada, Akihiro Yamaguchi, Kumiko Yamazaki, Shuichi Inohana, Satoshi Isagawa, Nobuyuki Kibune, Toshiya Satoyama, Shin Ichi Katsuda, Shinobu Suzuki, Masatoshi Watai, Naoto Hirose, Takahiro Mitsue, Hitoshi Shirakawa, Michio Komai

    研究成果: Article査読

    28 被引用数 (Scopus)

    抄録

    The rhizome of Kaempferia parviflora has been used in traditional Thai medicine. In this study, we identified and compared specific compounds from the hexane extract of K. parviflora with those from other Zingiberaceous plants by using gas chromatography-mass spectrometry. We identified 5,7-dimethoxyflavone (DMF), 5-hydroxy-3,7,3′,4′- tetramethoxyflavone (TMF), estimated 3,5,7-trimethoxyflavone, 5-hydroxy-7,4′-dimethoxyflavone, 3,5,7,4′-tetramethoxyflavone, and investigated their anti-inflammatory effects in rat basophilic leukemia (RBL-2H3) cells stimulated with an IgE antigen or a calcium ionophore. We found that DMF and TMF more potently inhibited antigen-induced degranulation than did nobiletin, a well-known anti-inflammatory agent. In addition, compared to RBL-2H3 cells stimulated with a calcium ionophore, those treated with DMF and TMF showed more marked inhibition of the degranulation and the production and mRNA expression of inflammatory mediators. These results suggest that DMF and TMF inhibit an early step in the high-affinity IgE receptor signaling cascade rather than intracellular calcium release and protein kinase C activation.

    本文言語English
    ページ(範囲)851-860
    ページ数10
    ジャーナルBioscience, Biotechnology and Biochemistry
    78
    5
    DOI
    出版ステータスPublished - 2014

    ASJC Scopus subject areas

    • バイオテクノロジー
    • 分析化学
    • 生化学
    • 応用微生物学とバイオテクノロジー
    • 分子生物学
    • 有機化学

    フィンガープリント

    「Identification and evaluation of anti-inflammatory compounds from Kaempferia parviflora」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

    引用スタイル