Identification and characterization of multiple β-glucan binding proteins in the Pacific oyster, Crassostrea gigas

Naoki Itoh, Ryo Kamitaka, Keisuke G. Takahashi, Makoto Osada

研究成果: Article査読

18 被引用数 (Scopus)

抄録

The present study reports on the characterization of two cDNAs coding β-glucan binding proteins (βGBPs), designated as Cg-βGBP-1 and Cg-βGBP-2, from the Pacific oyster, Crassostrea gigas. Cg-βGBP-1 consists of 555 amino acid residues and possesses two possible integrin recognition sites. The other protein, Cg-βGBP-2, is composed of 447 amino acid residues without integrin recognition sites. Domain structures of both Cg-βGBPs are similar to other invertebrate βGBPs, but phylogenetic positions and major expression tissues for these proteins are different. Cg-βGBP-1 is expressed in circulatory hemocytes and Cg-βGBP-2 in digestive glands. Functional assays using recombinant proteins revealed that Cg-βGBP-2 enhanced the phenoloxidase (PO) activity of hemocyte suspensions under the presence of laminarin, but Cg-βGBP-1 did not show this enhancement. It is suggested that Cg-βGBPs in the Pacific oyster have evolved to obtain different immunological functions. Cg-βGBP-1 possibly evolved for hemocyte-related functions through integrin, and Cg-βGBP-2 for the PO activation system.

本文言語English
ページ(範囲)445-454
ページ数10
ジャーナルDevelopmental and Comparative Immunology
34
4
DOI
出版ステータスPublished - 2010 4 1

ASJC Scopus subject areas

  • 免疫学
  • 発生生物学

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