Identification and characterization of genes involved in the downstream degradation pathway of γ-hexachlorocyclohexane in Sphingomonas paucimobilis UT26

Ryo Endo, Mayuko Kamakura, Keisuke Miyauchi, Masao Fukuda, Yoshiyuki Ohtsubo, Masataka Tsuda, Yuji Nagata

研究成果: Article査読

57 被引用数 (Scopus)

抄録

Sphingomonas paucimobilis UT26 utilizes γ-hexachlorocyclohexane (γ-HCH) as a sole source of carbon and energy. In our previous study, we cloned and characterized genes that are involved in the conversion of γ-HCH to maleylacetate (MA) via chlorohydroquinone (CHQ) in UT26. In this study, we identified and characterized an MA reductase gene, designated linF, that is essential for the utilization of γ-HCH in UT26. A gene named linEb, whose deduced product showed significant identity to LinE (53%), was located close to linF. LinE is a novel type of ring cleavage dioxygenase that catalyzes the conversion of CHQ to MA. LinEb expressed in Escherichia coli transformed CHQ and 2,6-dichlorohydroquinone to MA and 2-chloromaleylacetate, respectively. Our previous and present results indicate that UT26 (i) has two gene clusters for degradation of chlorinated aromatic compounds via hydroquinone-type intermediates and (ii) uses at least parts of both clusters for γ-HCH utilization.

本文言語English
ページ(範囲)847-853
ページ数7
ジャーナルJournal of bacteriology
187
3
DOI
出版ステータスPublished - 2005 2

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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