Two new acyloin compounds were isolated from the thermophilic bacterium Thermosporothrix hazakensis SK20-1T. Genome sequencing of the bacterium and biochemical studies identified the thiamine diphosphate (TPP)-dependent enzyme Thzk0150, which is involved in the formation of acyloin. Through extensive analysis of the Thzk0150-catalyzed reaction products, we propose a putative reaction mechanism involving two substrates: 4-methyl-2-oxovalerate as an acyl donor and phenyl pyruvate as an acyl acceptor. Discovery from a thermophile: Two new acyloins with R configurations at their chiral centers were isolated from the thermophilic bacterium Thermosporothrix hazakensis SK20-1T. These molecules were synthesized from two keto acid substrates by TPP-dependent Thzk0150 enzyme-catalyzed Cï£ ¿C bond condensation.
ASJC Scopus subject areas