Identification and biosynthesis of new acyloins from the thermophilic bacterium thermosporothrix hazakensis SK20 - 1T

Jin Soo Park, Noritaka Kagaya, Junko Hashimoto, Miho Izumikawa, Shuhei Yabe, Kazuo Shin-Ya, Makoto Nishiyama, Tomohisa Kuzuyama

研究成果: Article査読

14 被引用数 (Scopus)

抄録

Two new acyloin compounds were isolated from the thermophilic bacterium Thermosporothrix hazakensis SK20-1T. Genome sequencing of the bacterium and biochemical studies identified the thiamine diphosphate (TPP)-dependent enzyme Thzk0150, which is involved in the formation of acyloin. Through extensive analysis of the Thzk0150-catalyzed reaction products, we propose a putative reaction mechanism involving two substrates: 4-methyl-2-oxovalerate as an acyl donor and phenyl pyruvate as an acyl acceptor. Discovery from a thermophile: Two new acyloins with R configurations at their chiral centers were isolated from the thermophilic bacterium Thermosporothrix hazakensis SK20-1T. These molecules were synthesized from two keto acid substrates by TPP-dependent Thzk0150 enzyme-catalyzed Cï£ ¿C bond condensation.

本文言語English
ページ(範囲)527-532
ページ数6
ジャーナルChemBioChem
15
4
DOI
出版ステータスPublished - 2014 3 3
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 分子医療
  • 分子生物学
  • 有機化学

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