Hyperreactivity of aortic smooth muscle to serotonin is related to the presence of atheroma in watanabe heritable hyperlipidaemic rabbits

Hirofumi Tagawa, Hitonobu Tomoike, Wataru Mitsuoka, Shinji Satoh, Takeshi Kuga, Hiroaki Shimokawa, Motoomi Nakamura, Akira Takeshita

研究成果: Article査読

13 被引用数 (Scopus)

抄録

Objective: The aim was to elucidate the contribution of atheromatous plaque to alterations of smooth muscle contraction to vasoconstrictive agents, by examining vasoreactivity of vascular smooth muscle from the thoracic aorta of 10-13 month old Watanabe heritable hyperlipidaemic rabbits. Methods: From the same vascular ring of the lower thoracic aorta, a pair of small medial smooth muscle strips was prepared from the sites beneath the atheroma (atherosclerotic medial muscle strip) and from those beneath the plaque-free intima (normal medial muscle strip), and isometric tension was measured. Results: Contractions to 118 mM KC1, histamine (30 nM to 10 μM), and noradrenaline (3 nM to 0.3 μM) were similar between atherosclerotic and the normal medial muscle strip. The ED50 to serotonin was 49(SD 28) and 116(66) nM (p<0.05, n = 7) and the maximum tension to serotonin was 125(29)% and 82(29)% of that induced by 118 mM KC1 (p<0.01, n = 7) in atherosclerotic and normal medial muscle strip, respectively. Serotonin specific hyperreactivity of the atherosclerotic strip disappeared in Ca2+-free solution or in the presence of 10 μM H-7, an inhibitor of protein kinase C. After incubation with 0.1 μM phorbol 12,13-dibutyrate, an activator of protein kinase C, the isometric contractions induced by Ca2+ were significantly greater in atherosclerotic than in normal medial muscle strip. Conclusions: These results indicate that medial smooth muscle located beneath the atheroma is specifically hyperreactive to serotonin and that altered protein kinase C activity may explain in part the augmented response to serotonin.Cardiovascular Research 1993;27:2164-2169.

本文言語English
ページ(範囲)2164-2169
ページ数6
ジャーナルCardiovascular Research
27
12
DOI
出版ステータスPublished - 1993 12

ASJC Scopus subject areas

  • 生理学
  • 循環器および心血管医学
  • 生理学(医学)

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