Hyperbaric oxygen therapy improves early posttransplant islet function

Naoaki Sakata, Nathaniel K. Chan, Robert P. Ostrowski, John Chrisler, Pete Hayes, Sonny Kim, Andre Obenaus, John H. Zhang, Eba Hathout

研究成果: Article査読

27 被引用数 (Scopus)

抄録

Objective: This study investigates the therapeutic potential of hyperbaric oxygen therapy (HBO) in reducing hypoxia and improving engraftment of intraportal islet transplants by promoting angiogenesis.Methods: Diabetic BALB/c mice were transplanted with 500 syngeneic islets intraportally and received six consecutive twice-daily HBO treatments (n = 9; 100% oxygen for 1 h at 2.5 atmospheres absolute) after transplantation. Dynamic contrast-enhanced magnetic resonance imaging (DCE MRI) was used to assess new vessel formation at postoperative days (POD) 3, 7, and 14. Liver tissue was recovered at the same time points for correlative histology, including: hematoxylin and eosin, hypoxia-inducible factor (HIF1α), Terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick end labeling (TUNEL), vascular endothelial growth factor (VEGF), and von Willebrand Factor immunohistochemistry.Results: HBO therapy significantly reduced HIF-1α, TUNEL and VEGF expression in islets at POD 7. In the non-HBO transplants, liver enhancement on DCE MRI peaked at POD 7 consistent with less mature vasculature but this enhancement was suppressed at POD 7 in the HBO-treated group. The number of new peri-islet vessels at POD 7 was not significantly different between HBO and control groups.Conclusion: These results are consistent with a hyperbaric oxygen-mediated decrease in hypoxia that appeared to enhance vessel maturation in the critical days following intraportal islet transplantation.

本文言語English
ページ(範囲)471-478
ページ数8
ジャーナルPediatric Diabetes
11
7
DOI
出版ステータスPublished - 2010 11

ASJC Scopus subject areas

  • 内科学
  • 小児科学、周産期医学および子どもの健康
  • 内分泌学、糖尿病および代謝内科学

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