HVJ-E/importin-β hybrid vector for overcoming cytoplasmic and nuclear membranes as double barrier for non-viral gene delivery

Takeshi Kawazu, Hiroyuki Kanzaki, Atsushi Uno, Hideki Azuma, Takeshi Nagasaki

研究成果: Article査読

8 被引用数 (Scopus)

抄録

In order to enhance the nuclear import of the transgene, we prepared plasmid DNA/importin-β conjugates consisting of biotinylated poly(ethylenimine)s and recombinant streptavidin-fused importin-β. Hemagglutinating virus of Japan-envelope vector containing the PEI polyplex/importin-β conjugate showed high transfection efficiency not only in vitro but also in vivo. We showed that novel HVJ-E/importin-β-conjugated PEI polyplex hybrid vector could overcome plasma and nuclear membrane barriers to achieve effective transfection.

本文言語English
ページ(範囲)519-524
ページ数6
ジャーナルBiomedicine and Pharmacotherapy
66
7
DOI
出版ステータスPublished - 2012 10月
外部発表はい

ASJC Scopus subject areas

  • 薬理学

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