Humanized prion protein knock-in by Cre-induced site-specific recombination in the mouse

Tetsuyuki Kitamoto, Kenji Nakamura, Kazuki Nakao, Satoshi Shibuya, Ryong Woon Shin, Yoichi Gondo, Motoya Katsuki, Jun Tateishi

研究成果: Article査読

24 被引用数 (Scopus)

抄録

To establish humanized mice with a knock-in (gene replacement) technique, we constructed a targeting vector which consists of the human prion protein and the loxP sequences. The introduced human prion protein with the loxP system in the embryonic stem cells was transmitted through the mouse germ line. Transient expression of Cre recombinase in the fertilized eggs resulted in the prion protein humanized mice. The Cre-loxP-mediated gene replacement is a simple and efficient method which is generally applicable to make humanized animal models.

本文言語English
ページ(範囲)742-747
ページ数6
ジャーナルBiochemical and biophysical research communications
222
3
DOI
出版ステータスPublished - 1996 5 24

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

フィンガープリント 「Humanized prion protein knock-in by Cre-induced site-specific recombination in the mouse」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル