Human long-chain acyl-CoA synthetase: Structure and chromosomal location

Takaaki Abe, Takahiro Fujino, Ryuichi Fukuyama, Shinsei Minoshima, Nobuyoshi Shimizu, Hiroyuki Toh, Hiroyuki Suzuki, Tokuo Yamamoto

研究成果: Article査読

50 被引用数 (Scopus)

抄録

A complementary DNA clone encoding the entire human long-chain acyl-CoA synthetase was isolated and the total 698-amino acid sequence was deduced. The amino acid sequence of human long-chain acyl-CoA synthetase shows 84.9% identity to that of rat long-chain acyl-CoA synthetase. The nucleotide sequences of the protein coding regions between human and rat long-chain acyl-CoA synthetase mRNAs are highly conserved (85.6%), whereas those of the 3′ untranslated regions are less conserved (72%). The location of the human long-chain acyl-CoA synthetase gene was identified on chromosome 4 by spot hybridization of flow-sorted chromosomes. Computer-assisted homology search revealed a significant similarity of the enzyme with the enzymes of the luciferase family. Based on this similarity, the structure of human long-chain acyl-CoA synthetase can be divided into five domains: the N-terminus, two domains similar to those in enzymes of the luciferase family, a long gap region between the similar domains and the C-terminus.

本文言語English
ページ(範囲)123-128
ページ数6
ジャーナルJournal of biochemistry
111
1
DOI
出版ステータスPublished - 1992 1

ASJC Scopus subject areas

  • 生化学
  • 分子生物学

フィンガープリント

「Human long-chain acyl-CoA synthetase: Structure and chromosomal location」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル