Human immunodeficiency virus type 1 intergroup (M/O) recombination in Cameroon

Jun Takehisa, Léopold Zekeng, Eiji Ido, Yumi Yamaguchi-Kabata, Innocent Mboudjeka, Yosuke Harada, Tomoyuki Miura, Lazare Kaptué, Masanori Hayami

研究成果: Article査読

122 被引用数 (Scopus)

抄録

Here we describe, for the first time, recombinants between two highly divergent major groups of human immunodeficiency virus type 1 (HIV-1), M and O, within a Cameroonian woman infected with three different HIV-1 strains, a group O virus, a subtype D virus, and a recently reported IBNG (A/G)-like recombinant virus. Using nested extra-long PCR amplification, we sequenced from the pol region to the env region including accessory genes of the viral genome obtained from the patient's uncultured peripheral blood mononuclear cells and examined the phylogenetic position of each gene. Compared with sequential blood samples obtained in 1995 and 1996, there were multiple segmental exchanges between three HIV-1 strains (O, D, and IBNG) and all the recombinants appeared to be derived from a common M/O ancestor. Importantly, recombination between groups M and O occurred, even though the homology between these two groups is 69, 76, 68, and 55% in the gag, pol, vif-vpr, and env regions, respectively. Recombination between strains with such distant lineages may contribute substantially to generating new HIV-1 variants.

本文言語English
ページ(範囲)6810-6820
ページ数11
ジャーナルJournal of virology
73
8
DOI
出版ステータスPublished - 1999
外部発表はい

ASJC Scopus subject areas

  • 微生物学
  • 免疫学
  • 昆虫科学
  • ウイルス学

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