Human geranylgeranyl diphosphate synthase: cDNA cloning and expression

Tsuyoshi Kuzuguchi, Yuiko Morita, Ikuko Sagami, Hiroshi Sagami, Kyozo Ogura

研究成果: Article査読

43 被引用数 (Scopus)

抄録

Geranylgeranyl diphosphate (GGPP) synthase (GGPPSase) catalyzes the synthesis of GGPP, which is an important molecule responsible for the C20- prenylated protein biosynthesis and for the regulation of a nuclear hormone receptor (LXR·RXR). The human GGPPSase cDNA encodes a protein of 300 amino acids which shows 16% sequence identity with the known human farnesyl diphosphate (FPP) synthase (FPPSase). The GGPPSase expressed in Escherichia coli catalyzes the GGPP formation (240 nmol/min/mg) from FPP and isopentenyl diphosphate. The human GGPPSase behaves as an oligomeric molecule with 280 kDa on a gel filtration column and cross-reacts with an antibody directed against bovine brain GGPPSase, which differs immunochemically from bovine brain FPPSase. Northern blot analysis indicates the presence of two forms of the mRNA.

本文言語English
ページ(範囲)5888-5894
ページ数7
ジャーナルJournal of Biological Chemistry
274
9
DOI
出版ステータスPublished - 1999 2 26

ASJC Scopus subject areas

  • 生化学
  • 分子生物学
  • 細胞生物学

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