Human-derived corneal epithelial cells expressing cell cycle regulators as a new resource for in vitro ocular toxicity testing

Tomokazu Fukuda, Ryo Gouko, Takahiro Eitsuka, Ryusei Suzuki, Kohei Takahashi, Kiyotaka Nakagawa, Eriko Sugano, Hiroshi Tomita, Tohru Kiyono

研究成果: Article査読

5 被引用数 (Scopus)

抄録

The Draize test has been used on rabbits since the 1960s to evaluate the irritation caused by commercial chemicals in products such as cosmetics or hairdressing products. However, since 2003, such tests, including the Draize test for cosmetics, have been prohibited in European countries because they are considered problematic to animal welfare. For this reason, replacement of in vivo methods with the alternative in vitro methods has become an important goal. In this study, we established a corneal epithelial cell line co-expressing a mutant cyclin-dependent kinase 4 (CDK4), Cyclin D1, and telomerase reverse transcriptase (TERT). The established cell line maintained its original morphology and had an enhanced proliferation rate. Furthermore, the cells showed a significant, dose-dependent decrease in viability in an irritation test using glycolic acid and Benzalkonium chloride. These cells can now be shared with toxicology scientists and should contribute to increasing the reproducibility of chemical testing in vitro.

本文言語English
論文番号587
ジャーナルFrontiers in Genetics
10
JUN
DOI
出版ステータスPublished - 2019

ASJC Scopus subject areas

  • 分子医療
  • 遺伝学
  • 遺伝学(臨床)

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