Human airway submucosal glands augment eosinophil chemotaxis during rhinovirus infection

E. Furukuwa, T. Ohrui, M. Yamaya, T. Suzuki, H. Nakasato, T. Sasaki, A. Kanda, H. Yasuda, H. Nishimura, H. Sasaki

研究成果: Article査読

17 被引用数 (Scopus)

抄録

Background: Asthma exacerbations are frequently associated with rhinovirus (RV) infections. However, the contribution of airway submucosal gland (SMG) to exacerbations of asthma in RV respiratory infection has not been studied. Objective: This study was undertaken to examine whether RV-infected human respiratory SMG cells produce pro-inflammatory cytokines and chemokines for eosinophils, and augment eosinophil transmigration across human airway epithelium. Methods: We infected cultured human tracheal SMG cells with RV14, collected culture media at 1, 3, and 5 days after infection, and measured the chemotactic activity for eosinophils in the culture supernatant using a 48-well microchemotaxis chamber and a 51Cr-labelled eosinophil transmigration assay. Results: Exposing a confluent human tracheal SMG cell monolayer to RV14 consistently led to infection. Human SMG cells with RV infection secreted soluble factors activating human eosinophil chemotaxis into the culture supernatant in a time-dependent manner, and the culture supernatant significantly augmented the transmigration of 51Cr-labelled eosinophils through human airway epithelial cell layers from the basal to mucosal side. These effects were completely abolished by a mixture of a monoclonal antibody regulated on activation, normal T cells expressed and secreted (RANTES) and an antibody to granulocyte macrophage-colony stimulating factor (GM-CSF). Conclusion: These results suggest that human respiratory SMG cells may augment eosinophil transmigration across the airway epithelium through the secretion of RANTES and GM-CSF after RV infection, and may contribute to exacerbations of asthma.

本文言語English
ページ(範囲)704-711
ページ数8
ジャーナルClinical and Experimental Allergy
34
5
DOI
出版ステータスPublished - 2004 5月

ASJC Scopus subject areas

  • 免疫アレルギー学
  • 免疫学

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