Hrp48, a Drosophila hnRNPA/B homolog, binds and regulates translation of oskar mRNA

Tamaki Yano, Sonia López de Quinto, Yasuhisa Matsui, Anna Shevchenko, Andrej Shevchenko, Anne Ephrussi

研究成果: Article査読

98 被引用数 (Scopus)

抄録

Establishment of the Drosophila embryonic axes provides a striking example of RNA localization as an efficient mechanism for protein targeting within a cell. oskar mRNA encodes the posterior determinant and is essential for germline and abdominal development in the embryo. Tight restriction of Oskar activity to the posterior is achieved by mRNA localization-dependent translational control, whereby unlocalized mRNA is translationally repressed and repression is overcome upon mRNA localization. Here we identify the previously reported oskar RNA binding protein p50 as Hrp48, an abundant Drosophila hnRNP. Analysis of three hrp48 mutant alleles reveals that Hrp48 levels are crucial for polarization of the oocyte during mid-oogenesis. Our data also show that Hrp48, which binds to the 5′ and 3′ regions of oskar mRNA, plays an important role in restricting Oskar activity to the posterior of the oocyte, by repressing oskar mRNA translation during transport.

本文言語English
ページ(範囲)637-648
ページ数12
ジャーナルDevelopmental cell
6
5
DOI
出版ステータスPublished - 2004 5

ASJC Scopus subject areas

  • 分子生物学
  • 生化学、遺伝学、分子生物学(全般)
  • 発生生物学
  • 細胞生物学

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